A Study on the Wastewater Treatment from Antibiotic Production

Wastewater from cephalosporin antibiotic production with high bio-toxicity is hard to degrade, and could cause great harm to environment and human being. In the present paper, wastewater from cephalosporin production was processed with biochemical treatments as hydrolytic acidification, Up-flow Anaerobic Sludge Bed(UASB), Sequencing Batch Reactor Activated Sludge Process(SBR), biological activated carbon process(BAC). Among them, hydrolytic acidification could efficaciously enhance the biodegradability of wastewater, and greatly increase effects of the subsequent anaerobic-aerobic treatment. The final BAC process could effectively eliminate chemical oxygen demand (COD) and chroma of wastewater treated by aerobic treatment, where COD attained below 100mg/L and chroma was 40. Therefore, wastewater after the previous treatments basically conformed to the discharge standard of “ Integrated wastewater discharge standard “(GB8978-1996).


INTRODUCTION
The growing use of pharmaceuticals such as antibiotics worldwide, classified as the so-called emerging pollutants, has become a new environmental problem, which has raised great concern among scientists in the last few years.Wastewater from cephalosporin antibiotic production has complicated components, where organic substances and soluble or colloid solid substances are all at a high level with a great deal of suspended matter, and pH value varied often, contains biological toxicity substances of nonbiodegradable and bacteriostatic antibiotics.In the present paper, wastewater from cephalosporin production was processed with biochemical treatments as follows: hydrolytic acidification-UASB-SBR-BAC process, which guaranteed that wastewater basically conformed to the discharge standard of " Integrated wastewater discharge standard "(GB8978-1996).

Wastewater quality and outlet requirements
Wastewater was sampled from hospital discharge and a nearer factory of the district region.Relative data was listed in detail in Table 1.

Schematic process
Organic glass cylinder with an effective volume of 5L was used as hydrolytic acidification pool.UASB reactor with the height of 150cm, inner diameter of 8cm and effective volume of 5L, was kept at constant temperature of 37! by jacket water bath adjusted by temperature-control relay and heater.Each vessel was undertaken at a manner of continuous influent, water flew into hydrolytic acidification cylinder and UASB reactor through the bottom feed inlet and flew out from the top.Biogas resulted from UASB reactor was collected by gas trap hood.

Hydrolytic acidification
Using hydrolytic and acid-producing bacteria reactions, un-degradable organic substances were decomposed into biodegradable matter with small molecule, which further enhanced the biodegradability and thus decreased the charge of following processes.

USAB (Up-flow Anaerobic Sludge Bed) reaction
Basic structure of UASB reactor was mainly composed of sludge bed, sludge suspension layer, precipitate zone, three-phase separator and intake system, and granular sludge in reaction zone was the key of this reactor.It is a new type and high effective wastewater treatment equipment, which altered the traditional and laggard treatment of anaerobic fermentation, and have novel insight in the design of inlet manner, influent distribution system, agitated mixing and three-phase separator and intake system, and thus could be regarded as an ideal equipment for dealing with wastewater of high, medium and low pollution level.During the

SBR (Sequencing Batch Reactor) Method
SBR method, namely sequencing batch reactor, is a regeneration and modification of early filling-and-emptying reactor.The obvious predominance of SBR includes as follows: simple technological process, low cost of capital construction and performance; reaction phase is an ideal plug flow process with strong impetus resulted from biochemical reaction and good efficacy; flexible performance manner, good effect of deamination phosphorus removing, and optimal technology for preventing sludge from swelling; resistance to shock loading, and good capability of dealing with toxic or high concentration organic wastewater.SBR was used as subsequent process of anaerobic treatment, in order to assure that the whole effects of system attained the discharge standard.SBR reactor was a cylinder made of 5mm organic glass with total volume of 4.5L, diameter of 140mm and effective height of 293mm.Aerobic sludge sampled from secondary sedimentation tank of a sewage treatment factory was cultured and acclimated in SBR reactor.8d later, system performance was basically stable, and the color of sludge appeared deep tan with larger floccule, clear margin and better settling property, which indicated that sludge culture had succeeded.If water inflow was too strong, heterotrophic bacteria in reactor would propagated largely due to sufficient nutrition, while nitrobacteria propagated slowly with small specific growth rate, and accounted for less and less proportion in sludge.Moreover, both bacteria would compete for the substrates and dissolved oxygen, which had inhibitory effect on the generation of nitrobacteria.Therefore, each water inflow should not be above 1.5L, and under such situation, sludge load of reactor was 0.40kgCOD/ (kgMLSS•d), and COD removal was 80%~85%.Results were depicted in Figure 4.

BAC (Biological Activated Carbon) Method
BAC technology used activated carbon with huge specific surface area and developed void structure as carriers for aggregation, propagation and growth of microorganisms, and under the condition of moderate temperature and nutrition, exerted microbiological degradation simultaneously.Such water treatment technology was also called BAC method.Interaction of BAC granule, microorganism and water pollutants (matrix) and dissolved oxygen was involved in the process of wastewater treatment by BAC.From micro perspective, synergistic effect of BAC adsorption and microbiological degradation was not ordinary superposition of both.In the present paper, adsorption capacity of BAC was investigated, where granule fragmentary carbon with high adsorption capacity for organic substances was selected as biological carrier, meanwhile diluted pig manure sludge was undertaken to aerobic acclimatization and culture in lab scale, which was applied as biological source to prepare BAC for investigation.5d later, through the observation of microscope, a layer biofilm was coated in the surface of BAC, which indicated that preparation of BAC has tended to be mature and could be undertaken to treat with wastewater from cephalosporin production.Wastewater after processes of hydrolytic acidification-UASB-SBR was added to BAC column, in a manner of lower inlet and upper outlet, influents and effluents COD, chroma and removal was presented in Figure 5. Results showed that BAC advanced treatment could effectively eliminate the COD and chroma of wastewater treated by aerobic treatment, where COD attained below 100mg/L and chroma was 40.

CONCLUSION
In the normal process of reactor, COD removal of hydrolytic acidification and UASB reactor effluents was kept 36%~55% and 80%~90%, respectively.Sludge load of SBR reactor was 0.40kg COD/ (kgmlSS•d), and COD removal was 80%~85%.BAC advanced treatment could effectively eliminate the COD and chroma of wastewater treated by aerobic treatment, where COD attained below 100mg/L and chroma was 40.Therefore, wastewater basically conformed to the discharge standard of "Integrated wastewater discharge standard"(GB8978-1996).

Table 1 : Wastewater original quality and outlet requirements Indices pH
COD/mg/l BOD/mg/l SS/mg/l Chrome