Green Technical Methods for Treatment of Waste Water Using Microalgae and Its Application in the Management of Natural Water Resources: a Review

AbSTRAcT Water covers 71% of the Earth's surface, and is vital for all known forms of life.But only 2.5% of the Earth's water is freshwater. Due to industrialization and Urbanization it is becoming more polluted and risk of this polluted water consumption and its sanitation problem is increasing day to day in most of the developing countries, so it has become anessential need for today's environment to protect water from getting polluted or develop its cost effective remedial method for its protection. Literature survey was done to find out the new, low-cost waste water treatment methods in which we had found that Microalgae has the natural wastewater treatment properties. It has the self cleansing power due to which it abstracts Nitrate 99%, sulphate 84% and Phosphate 73% for its growth and development. During their growth they trap sun light and CO 2 from the environment for their photosynthesis. In the mean time of review of literature we had found that waste water treatment using microalgae has number of positive applications over the conventional methods as it is useful in Wastewater treatment, CO2 sequestration, Cost effective, Sanitation and also in the production of renewable sources of energy such as Biodiesel, Biofuel, Glycerol, Methane gas, Hydrogen gas, Biofertilizers etc. The overall review has concluded that this Green technical method for treatment of municipal waste water using microalgae should be applied in all developing and developed countries for wastewater treatment so as to protect the environmental pollution causing due to waste water from industrial and Societies effluents.

Water is one of the most impor tant substances on earth.All plants and animals must have water to survive.If there was no water there would be no life on earth 1 .It covers 71% of the Earth's surface, and is vital for all known forms of life.But only 2.5% of the Earth's water is fresh water.Rapid urbanization and industrialization releases enormous volumes of wastewater, which is increasingly utilized as a valuable resource for irrigation in urban and peri-urban agriculture.It drives significant economic activity, supports countless livelihoods particularly those of poor farmers, and substantially changes the water quality of natural water bodies 2 .Due to industrialization and Urbanization it is becoming more polluted and risk of this polluted water consumption and its sanitation problem is increasing day to day in most of the developing countries.This growing problem of water scarcity has significant negative influence on economic development, human livelihoods, and environmental quality throughout the world 3 .So it has become anessential need for today's environment to protect water from getting polluted or develop its cost effective remedial method for its protection.
So the new green technical methods are being introduced to overcome the conventional methods of waste water treatment [12][13][14][15] .The present study is related with new green technical methods which are proving them to be superior over the conventional methods; out of them low cost waste water treatment using microalgae is the potential one.Literature survey was done to find out the new methods of waste water treatment using microalgae and their development till now and to find out its application in management of natural water resources.

background
The concept of wastewater treatment using microalgae was firstly proposed by Oswald in the 1950 16,17 and the concept was later expanded to propose use of this system for energy production through harvesting and utilization of algal biomass 18 .Shirai F, et.al. in the year 1998 who investigate the treatment of Soy Sauce effluent including four types of microalgae 19 in which they applied this method for the fermentation of ethanol from the biomass which was obtained by harvesting microalgae.After that so many scientist turned their research work to treat various industrial and municipal waste water using different types of microalgae [20][21][22][23][24][25][26] .

Green Aspects of Wastewater treatment using Microalgae
Wastewater treatment using microalgae shows its potential ability towards the greenery ofenvironment and its suitability for applying it in green technical methods.It obeys most of the basic principles of green chemistry which was given by Paul T. Anastas 27 .The green applications of this technique are listed below.1.
Removal of Nutrient pollutants and Heavy Metals 2.
Low energy requirement 4.
Production of Useful Biomass 5.
Reduction in sludge Formation 6.
Algae contain more than 50% of oil in its biomass 7.
They provide much higher yields of biomass and fuel 8.
They can be grown under condition which is unsuitable for conventional crop production.

Nutrient removal capability of Microalgae
A major requirement in wastewater treatment is the removal of nutrients and toxic metals to acceptable limits prior to discharge and reuse 28 .Algae are autotrophs, i.e. they can synthesize organic molecules themselves from inorganic nutrients.A stoichiometric formula for the most common elements in an average algal cell is C106H181O45N16P, and the elementsshould be present in these proportions in the medium for optimal growth 29 .Microalgae have been proven to be efficient in removing nitrogen, phosphorus, and toxic metals from a wide variety of wastewaters 30,31,32 .There are extensive studies of algae growth in municipal 33,34 , agricultural 35,36 , and industrial wastewaters 37,38 .Substantial amounts of nutrient removal and algae biomass production were obtained in these studies.Hence, controlled microalgae cultivation shows promise as a potential biological treatment method for wastewater.This integrated wastewater treatment and biofuel production system can thus benefit the community as well as the environment 39 .Nitrogen Removal Nitrogen is a critical nutrient required in the growth of all organisms.Organic nitrogen is found in a variety of biological substances, such as peptides, proteins, enzymes, chlorophylls, energy transfer molecules (ADP, ATP), and genetic materials (RNA, DNA) 40 .Organic nitrogen is derived from inorganic sources including nitrate (NO3_), nitrite (NO2_), nitric acid (HNO3), ammonium (NH4þ), ammonia (NH3), and nitrogen gas (N2).Micro-algae play a key role in converting inorganic nitrogen to its organic form through a process called assimilation.In addition, cyanobacteria are capable of converting atmospheric nitrogen into ammonia by means of fixation.

phosphorous Removal
Phosphorus is also a key factor in the energy metabolism of algae and is found in nucleic acids, lipids, proteins, and the intermediates of carbohydrate metabolism.Inorganic phosphates play a significant role in algae cell growth and metabolism.Phosphates are transferred by energized transport across the plasma membrane of the algal cell.Not only are inorganic forms of phosphorus utilized by microalgae, but some varieties of algae are able to use the phosphorus found in organic esters for growth 41 .

other Nutrients
Although, nitrogen and phosphorous are the two main nutrients of concern in eutrophication, being limiting factors in most growth scenarios 42 , other micronutrients, including silicon and iron, can affect the abundance of phytoplankton communities 43 .How-ever, many of the micronutrients are toxic to most algae species at high concentrations.Some of them also form precipitates with other essential elements and reduce their availability.However some algae strains are particularly tolerant to heavy metals and their potential to absorb metals has been demonstrated 44 .

Low energy requirement
Traditional wastewater treatment processes involve the high energy costs of mechanical aeration to provide oxygen to aerobic bacteria to consume the organic compounds in the wastewater, whereas in algae based wastewater treatment, algae provides the oxygen for aerobic bacteria.Aeration is an energy intensive process, accounting for 45 to 75% of a wastewater treatment plant's total energy costs.Algae provide an efficient way to consume nutrients and provide the aerobic bacteria with the needed oxygen through photosynthesis.Roughly one kg of BOD removed in an activated sludge process requires one kWh of electricity for aeration, which produces one kg of fossil CO2 from power generation (Oswald, 2003).By contrast, one kg of BOD removed by photosynthetic oxygenation requires no energy inputs and produces enough algal biomass to generate methane that can produce one kWh of electric power (Oswald, 2003).

cost effective
It has been shown to be a more cost effective way to remove biochemicaloxygen demand, pathogens, phosphorus and nitrogen than activated sludge process and other secondary treatment processes (Green et al., 1996) 45

production of Useful Algal biomass
The resulting algae biomass is a source of useful products such as biodiesel.Previous research in the early 1990's by the National Renewable Energy Laboratory (NREL) showed that under controlled conditions algae are capable of producing 40 times the amount of oil for biodiesel per unit area of land, compared to terrestrial oilseed crops such as soy and canola (Sheehan et al., 1998)  46 .However, their results also showed that large-scale algae cultivation for energy production was uneconomical at that time and suggested future research into waste -stream integration (Sheehan et al., 1998).It is hoped that the economics will be ultimately improved by combining biodiesel feedstock production with agricultural or municipal wastewater treatment and CO2 fixation.

Reductions in Sludge Formation
In conventional wastewater treatment systems the main aim is to minimize or eliminate the sludge.Industrial effluents are conventionally treated using a variety of hazardous chemicals for pH correction, sludge removal, colour removal and odour removal.Extensive use of chemicals for effluent treatment results in huge amounts of sludge which forms the so called hazardous solid waste generated by the industry and finally disposed by depositing them in landfills.In algae wastewater treatment facilities, the resulting sludge with algal biomass is energy rich which can be further processed to make biofuel or other valuable products such as fertilizers.Algal technology avoids use of chemicals and the whole process of effluent treatment is simplified.There is considerable reduction in sludge formation 15 .

The GhG emission Reduction
Carbon, in the form of carbon dioxide, may be fixed from the atmosphere and industrial exhaust gases through the photosynthetic activity of autotrophic microalgae.Carbon can be also utilized in the form of soluble carbonates for cell growth, either by direct uptake or conversion of carbonate to free carbon dioxide through carbo anhydrase activity.The use of algae to mitigate carbon dioxide from flue gases is another research focus and, if effective, could benefit both the environment and biofuel production 28 .
The Us Environmental Protection Agency (EPA) has specifically identified conventional wastewater treatment plants as major contributors to greenhouse gases 48 .Algae based waste water treatment also releases CO2 but the algae consume more CO2 while growing than that is being released by the plant, this makes the entire system carbon negative.

otherApplications
Algae can be used to make bioethanol and biobutanol and by some estimates can produce vastly superior amounts of vegetable oil, compared to terrestrial crops grown for the same purpose.Algae can be grown to produce Hydrogen.In 1942 a German researcher named Hans Gaffron, while working at the University of Chicago, observed that the algae he was studying, Chlamydomonas reinhardtii (a green algae), would sometimes switch from the production of Oxygen to the production of Hydrogen 47 .Algae can be grown to produce biomass, which can be burned to produce heat and electricity.

ReSULT
Microalgae have the natural wastewater treatment properties.It has the self cleansing power due to which it abstracts Nitrate around 90%, sulphate 80% and Phosphate 70% for its growth and development.During their growth they trap sun light and CO 2 from the environment for their photosynthesis.In the mean time of review of literature we had found that waste water treatment using microalgae has number of positive applications over the conventional methods as it is useful in Wastewater treatment, CO2 sequestration, Cost effective, Sanitation and also in the production of renewable sources of energy such as Biodiesel, Biofuel, Glycerol, Methane gas, Hydrogen gas, Biofertilizers etc.

coNcLUSIoNS
The overall review has concluded that this Green technical method for treatment of municipal waste water using microalgae should be applied in all developing and developed countries for wastewater treatment so as to protect the environmental pollution causing due to waste water from industrial and domestic effluents.