<p><span style="font-size:14px"><span style="font-family:Arial,Helvetica,sans-serif">Application of Chlorpyrifos on agricultural fields to protect crops against pests results in accumulation of it in soil and other environmental samples. The insecticide transform &nbsp;into <strong>&nbsp;</strong>3,5,6-Trichloro-2-Pyridinol (TCP) through hydrolysis in soil, which has got antimicrobial property and hence resists its degradation in natural condition. In the current findings, a bacterial isolate capable of mineralizing Chlorpyrifos without accumulation of TCP was isolated from agricultural soil by enrichment method. Based on Morphological, Biochemical Characterization and with Bergey&rsquo;s Manual comparision, the isolate was identified as <em>Staphylococcus sp</em>. The isolate was found to metabolize chlorpyrifos completely in Mineral salt medium with chlorpyrifos as the sole carbon source. No metabolites of chlorpyrifos were detected in Liquid Chromatography-Mass Spectroscopy (LC-MS) analysis after 7 days of incubation. The novelty of the outcome of the experiment relies on <em>Staphylococcus</em> sp.ES-2 in complete mineralization of chlorpyrifos which can be used as a potential bioaugmenting agent in the chlorpyrifos contaminated sites.</span></span></p>