<p><span style="font-size:14px"><span style="font-family:Arial,Helvetica,sans-serif"><span style="background-color:white"><span style="color:black">Gut analysis is the tool to understand the feeding patterns of fishes and is an important aspect of fisheries management. It also provides the basis for understanding trophic interactions in aquatic food webs and to investigate the most frequently consumed prey or to determine the relative importance of different food types to fish nutrition. In the present study the gut content analysis was performed in Garra, Gobi, Notopterus and Tilapia fishes collected from Tungabhadra upper irrigation channel at Ballari, Karnataka.</span></span>Bacillariophyceae showed maximum number in all the four fish species. Over all it showed 40% followed by Detritus (30%), Chlorophyceae (17%), Cyanophyceae(7%) and Zooplankton (6%). Among fishes Garrashowed maximum food items (2272) followed by <em>Glossogobiusgiuris</em>(1538), <em>Notopterusnotopterus&nbsp;</em>(996) and <em>Oreochromismossambicus</em> (769). The relative abundance of food items in the guts also revealed the&nbsp;<em>Garragotylastenorhynchus&nbsp;<em>Oreochromismossambicus</em>. The variation is due to availability of foodorganisms during the study period and anthropogenic influence on channel water.</em></span></span></p>