The excessive growth of invasive weeds causes adverse economic and environmental effects. In the present study, invasive weed Calotropis gigantea was pyrolyzed under optimized parameters of 450° and 50-100 mm particle size for 1.00 hour of reaction time for biochar production. The biochar was characterized by the presence of a high carbon content of 64.65% and low H/C and O/C molar ratios of 0.08 and 0.15, respectively. The biochar was observed with high surface area of 99.91m2/g and pore volume of 0.0398cm3/g along with mineral fractions such as K-1.33%, Na-1.17%, Mg-1.05%. Strong FTIR bands were observed at 1994.1 cm-1, 1110 cm-1, and 745 cm-1, representing allenes (R 2C=C=CR 2), aryl alkyl ethers (R – O – R), and aromatic (C–H) bending. All these parameters indicate its potential in the applications such as carbon sequestration, climate change mitigation, environment pollutants adsorption (both organic and inorganic), and soil improvement.