<p><span style="font-size:14px"><span style="font-family:Arial,Helvetica,sans-serif">The environment, in which organism exists, has a profound effect on the behavior of organism. Temperature and pH are the two major abiotic factors governing the growth and activity of microbes. Microbes secrete number of degrading enzymes to degrade waste products. The microbes also have the ability of sorption the pollutants like heavy metals. Present works shows the analysis of microbes present in different environment of waste water of many industries and ponds. The fungal flora present in this waste water was incubated and isolated. Industrial water samples showed the pH range of 9-9.6, whereas the pond water sample showed the pH range of 7.5-8.8. High percentage of the organic matter was obtained in pond water (0.134%) as compared to industries waste water (0.045%). Industrial waste water showed the high Ca⁺² metal concentrations. The maximum number of fungal species (</span></span><span style="font-family:Arial,Helvetica,sans-serif"><span style="font-size:14px">Aspergillius</span><span style="font-size:14px"> </span><span style="font-size:14px">candidus</span><span style="font-size:14px">, A. </span><span style="font-size:14px">terres</span><span style="font-size:14px">, A. </span><span style="font-size:14px">fumigatus</span><span style="font-size:14px">, Humicola species and Talaromyces </span><span style="font-size:14px">emersonii</span></span><span style="font-size:14px"><span style="font-family:Arial,Helvetica,sans-serif">) were isolated from pond water because of high organic matter (in %) and low pH as compared to industrial waste water.</span></span></p>